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Orntipa Sethabutr, Leanne E. Unicomb, Ian H. Holmes, David N. Taylor, Ruth F. Bishop, Peter Echeverria, Serotyping of Human Group A Rotavirus with Oligonucleotide Probes, The Journal of Infectious Diseases, Volume 162, Issue 2, August 1990, Pages 368–372, https://doi.org/10.1093/infdis/162.2.368
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Abstract
Rotaviruses (RV) in stools ofchildren with diarrhea in Thailand were serotyped by monoclonal enzyme immunoassay (MEIA), and RNA extracted from these specimens were tested for hybridization with oligonucleotides constructed from the nucleotide sequences ofVP7 ofhuman serotypes 1, 2, 3, and 4. Of 178 specimens that contained RV-as identified with a monoclonal antibody to group ARV, 84% (149/178) hybridized with serotype-specific oligonucleotides, and 42% (74/178) were serotyped by MEIA (P < .001). Of the 74 specimens that were serotyped by MEIA, 92% (35/38) of type 1, 97% (34/35) of type 2, and the one type 4 RV hybridized with the HuGlAc, HuG2Ac, and HuG4Ac oligonucleotides, respectively. RV strains identified in children in Thailand in 1987 and 1988 to which a serotype could be assigned by either method were either type 1, type 2, or, less often, type 4. Testing RV for hybridization with oligonucleotides for genes encoding VP7 is an alternate method of determining RV serotypes.