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Helena Käyhty, Olli Mäkelä, Juhani Eskola, Leena Saarinen, Ilkka Seppälä, Isotype Distribution and Bactericidal Activity of Antibodies After Immunization with Haemophilus influenzae Type b Vaccines at 18–24 Months of Age, The Journal of Infectious Diseases, Volume 158, Issue 5, November 1988, Pages 973–982, https://doi.org/10.1093/infdis/158.5.973
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Abstract
The serum antibody response to Haemophilus influenzae type b capsular polysaccharide or its protein conjugate vaccine (PRP and PRP-D, respectively) was studied in 28 children initially immunized at the age of 24 mo with either vaccine and in 10children immunized for the third time with PRP-D at the age of 18 mo. The methods used were isotype-resolving enzyme immunoassay, Farr-type radioimmunoassay, and the in vitro bactericidal activity (BCA) test. Immunization with PRP evoked a higher proportion of IgA antibodies than did either the first or third dose of PRP-D, whereas the latter vaccine evoked a somewhat higher IgO response, but the differences were not statistically significant. In all groups the IgO antibody responses were predominantly IgG, with the mean proportions being 82.2%, 84.2%, and 65.9% in the PRP, first-dose PRP-D, and third-dose PRP-D groups, respectively. Postimmunization antibodies were functionally active in the BCA test.
