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Victor P. Berardi, Karen E. Weeks, Allen C. Steere, Serodiagnosis of Early Lyme Disease: Analysis of IgM and IgG Antibody Responses by Using an Antibody-Capture Enzyme Immunoassay, The Journal of Infectious Diseases, Volume 158, Issue 4, October 1988, Pages 754–760, https://doi.org/10.1093/infdis/158.4.754
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Abstract
We used an antibody-capture enzyme immunoassay (EIA) to evaluate the early antibody responses to Borrelia burgdorferi in paired sera from 30 patients with erythema chronicum migrans. During acute disease, 20 (67%) patients had elevated specific IgM responses, and by convalescence (one to four weeks after treatment), 28 (93%) patients had increased IgM or IgG responses. In acute specimens, elevated IgM responses correlated with disseminated infection; however, by convalescence, most patients with either localized or disseminated disease had positive tests. Among 133 control subjects, IgM cross-reactivity was observed in 4 of 37 patients with either Epstein-Barr virus or rickettsial infections, and false-positive IgG tests were seen in 8 of 28 patients with syphilis. With antibody-capture EIA, the diagnosis of Lyme disease can be confirmed in the majority of acutely ill patients and in almost all patients by convalescence.
- lyme disease
- herpesvirus 4, human
- syphilis
- acute disease
- antibody formation
- borrelia burgdorferi
- cross reactions
- erythema chronicum migrans
- rickettsia infections
- immunoglobulin g
- immunoglobulin m
- infections
- antibodies
- convalescence
- diagnosis
- igg antibody
- enzyme immunoassay
- false-positive results
- serum