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Ellena M. Peterson, Sandra L. Aarnaes, Robert N. Bryan, Jerry L. Ruth, Luis M. de la Maza, Typing of Herpes Simplex Virus with Synthetic DNA Probes, The Journal of Infectious Diseases, Volume 153, Issue 4, April 1986, Pages 757–762, https://doi.org/10.1093/infdis/153.4.757
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Abstract
Three single-stranded oligonucleotide probes, 22 bases long, homologous to unique regions of herpes simplex virus (HSV) types 1 (HSV-1) and 2 (HSV-2) and a region common to both were chemically synthesized with use of a modified phosphochloridite protocol. For hybridization experiments each probe was labeled with use of polynucleotide kinase and [γ-32P] ATP to a specific activity of ∼2 × 109 cpm/μg. Two hundred one clinical isolates of HSV (96 HSV-1 and 105 HSV-2) collected from vesicles in the mucocutaneous junction of the mouth or from the genital area were analyzed. There was a 99% (199 of 201) agreement between hybridization and monoclonal antibody typing; the two discrepant isolates of HSV-2 that were negative by monoclonal antibody typing were confirmed as HSV-2 by restriction endonuclease analysis. The probes detected between 104 and 105 HSV infectious units and from 150 to 600 HSV-infected Vero cells. No binding was detected between any ofthe three probes and isolates of cytomegalovirus, Epstein-Barr virus, and varicellazoster virus.