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Laurent Gutmann, Russell Williamson, A Model System to Demonstrate That β-Lactamase-Associated Antibiotic Trapping Could Be a Potential Means of Resistance, The Journal of Infectious Diseases, Volume 148, Issue 2, August 1983, Pages 316–321, https://doi.org/10.1093/infdis/148.2.316
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Abstract
Addition of β-Iactamaseto cultures of antibiotic-sensitive Escherichia coli protected the bacteria against lysis induced by either a hydrolyzable (cephalothin) or relatively nonhydrolyzable (ceftriaxone) cephalosporin. The later addition of a nonhydrolyzable, non-lysis-inducing β-Iactam antibiotic (oxacillin), which had a higher affinity for the β-Iactamase than ceftriaxone, allowed the reversal of the protection and the onset of lysis. These results suggest that trapping of the antibiotic by the enzyme, without significant hydrolysis, is a reversible process that may playa role in the resistance of some gram-negative bacteria to third-generation cephalosporins.