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D. E. Mosie, N. M. Zaldivar, E. Goldings, J. Mond, I. Scher, W. E. Paul, Formation of Antibody in the Newborn Mouse: Study of T-Cell-Independent Antibody Response, The Journal of Infectious Diseases, Volume 136, Issue Supplement_1, August 1977, Pages S14–S19, https://doi.org/10.1093/infdis/136.Supplement.S14
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Abstract
The ontogeny of immune responsiveness, as assayed by antibody formation in vitro, of mouse spleen lymphocytes to thymus-independent antigens is reviewed. Responsiveness to trinitrophenyl (TNP)-lipopolysaccharide and TNP-Brucella abortus appear soon after birth and one to two weeks before TNP-Ficoll or capsular polysaccharide of Streptococcus pneumoniae (SSS-III) elicits significant antibody formation. This hierarchy of responsiveness to antigens is also apparent in the CBA/N mutant mouse strain, which has a bone marrow-derived (B-) cell maturation arrest and fails to respond to either TNP-Ficoll or SSS-IlI. These findings are interpreted to suggest sequential maturation of different populations or lines of B-lymphocytes, each of which can respond to a defined class of thymus-independent antigens. The implication for vaccine use in humans is that a late-appearing subclass of B-cells may be req uired for adequate immune responses to polysaccharide antigens.