-
Views
-
Cite
Cite
Geoffrey Furness, Maria DeMaggio, The Growth Cycle of Mycoplasma mycoides var. mycoides, The Journal of Infectious Diseases, Volume 127, Issue 5, May 1973, Pages 563–566, https://doi.org/10.1093/infdis/127.5.563
Close - Share Icon Share
Abstract
Three strains of Mycoplasma mycoides var. mycoides were studied. Dark-ground microscopy of broth cultures showed some filamentous forms, but single cells predominated. The filaments disintegrated on agitation. Single cells passed through 0.3-µm pores but not 0.22µm pores of Millipore membranes. They were destroyed by shaking, sonication, storage at 2–4 C, and freezing and thawing. Ultraviolet-inactivation curves showed that single-cell suspensions contained both mononucleate and binucleate cells that could not be cloned into pure cultures of either type. They are considered part of a life cycle in which filaments are a characteristic but not a fundamental part. Electron micrographs showed that replicating cells varied in size from 0.15 µm to 4.5 µm in diameter. All multiplied by budding. Most synchronized cells produced one bud, but some produced two simultaneously, which confirmed that these cells were multinucleate and have an alternative mode of division in addition to binary fission.
