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PIER GIORGIO CROSIGNANI, ROBERT M. NAKAMURA, DAVID N. HOVLAND, DANIEL R. MISHELL, A Method of Solid Phase Radioimmunoassay Utilizing Polypropylene Discs, The Journal of Clinical Endocrinology & Metabolism, Volume 30, Issue 2, 1 February 1970, Pages 153–160, https://doi.org/10.1210/jcem-30-2-153
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Abstract
A new solid phase method of radioimmunoassay has been developed utilizing easily obtained, inexpensive discs of polypropylene. Study of the kinetics of binding of antibody to the discs revealed that binding capacity was related to pH as well as molarity of various buffers. In these solutions maximal binding is constantly achieved in 2 hr and persists in a stable state after lyophilization. This method has been used to assay serum HCG, LH and HPL. For serum LH the linear range of this assay method is from 4.2 to 52 μg/100 ml (radioimmunoassay, LER 907 standard) using NIH anti-HCG. For HPL the linear range is from 0.1 to 3 ng/ml of the 98% pure Lederle preparation. In contrast to the 3–5 days required for the double antibody method, this degree of sensitivity is obtained after only 18-hr incubation. Assay of HCG and HPL from the same serum samples obtained from 5 women at intervals from delivery to 3 days post partum confirmed that these hormones have different disappearance rates, the half-life being 20 min for HPL and 5 hr for HCG. Assay of LH in serum from menstruating women also yielded physiologic results, thus providing additional evidence for the specificity of the method.