COVER: Structure of HIRAN in complex with dsDNA

Structure of HIRAN bound to dsDNA is shown in the left panel. The strand of which 3′-end bound to HIRAN is colored in cyan and defined as the daughter strand. The complemental strand is shown as the parental strand colored in pink. Amino acid residues involved in interaction with DNA are shown by ball-and-stick models.

Superimposition of an ideal B-form DNA and the HIRAN-DNA complex is shown in the right panel. The ideal dsDNA is shown by a transparent model. HIRAN is shown by ribbon and semitransparent surface models. The 3′-end and −1 ∼ −3 nucleobases in the ideal dsDNA are labeled by grey. The arrow indicates movement of the 3′-OH from the original position in the ideal B-form DNA.

Hishiki A., Sato M., Hashimoto H. (2020) Structure of HIRAN domain of human HLTF bound to duplex DNA provides structural basis for DNA unwinding to initiate replication fork regression. J. Biochem. 167, 597–602