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Yuuki Okino, Yujin Inayoshi, Yusuke Kojima, Shunsuke Kidani, Hidenori Kaneoka, Akiko Honkawa, Hiroshi Higuchi, Ken-ichi Nishijima, Katsuhide Miyake, Shinji Iijima, Moloney murine leukemia virus integrase and reverse transcriptase interact with PML proteins, The Journal of Biochemistry, Volume 152, Issue 2, August 2012, Pages 161–169, https://doi.org/10.1093/jb/mvs063
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Abstract
Pull-down assay and co-immunoprecipitation of cell extracts in which the integrase or reverse transcriptase of Moloney murine leukemia virus was transiently expressed showed that both enzymes interacted with PML proteins. In infected cells, interaction between the integrase and PML was also observed. Transient expression of PIASy and SUMO proteins facilitated SUMOylation of the integrase but had no apparent effects on the interaction with PML. A FLAG-tagged integrase co-localized with PML protein possibly in the PML body. Knockdown of PML by small interfering RNA resulted in reduced viral cDNA levels and integration efficiency. This suggested that PML proteins activated reverse transcription.