Volume 100, Issue 8, August 2022
Featured Collection
Infographic
NC1184: Molecular Mechanisms Regulating Skeletal Muscle Growth and Differentiation
Review
Molecular and biochemical regulation of skeletal muscle metabolism
This review summarizes the major metabolic pathways in skeletal muscle and the associated regulatory pathways.
Satellite Cells
Thermal stress and selection for growth affect myogenic satellite cell lipid accumulation and adipogenic gene expression through mechanistic target of rapamycin pathway
The results of the current study provide valuable information on the development of temperature manipulation strategies to reduce the fat content in breast muscle satellite cells, which may decrease the incidence of structural defects and improve the breast meat quality of modern commercial poultry.
The initial delay to mitotic activity in primary cultures of equine satellite cells is reduced by combinations of growth factors
Combinations of growth factors are required for optimal muscle stem cell activity in vitro.
Effect of combined maternal and post-hatch dietary 25-hydroxycholecalciferol supplementation on broiler chicken Pectoralis major muscle growth characteristics and satellite cell mitotic activity
This study is the first of its kind and answers not only practical questions for the broiler industry regarding the possible benefits of combining maternal and post-hatch dietary 25OHD3 supplementation but simultaneously investigates the mode of action related to stimulation of broiler muscle growth. Although the combination of pre- and post-hatch dietary supplementation did not result in additive effects, inclusion of 25OHD3 in either the maternal or post-hatch diet stimulated a satellite cell (SC)-mediated breast muscle hypertrophic growth response.
Effects of in ovo injection of nicotinamide riboside on high-yield broiler myogenesis
A novel in ovo injection supplement influences high-yield broiler embryo myogenesis, also improves high-yield broiler embryo myogenesis.
Short Communication: Beta-adrenergic agonists alter oxidative phosphorylation in primary myoblasts
Beta-adrenergic agonist (β-AA) supplementation may improve the efficiency of muscle satellite cells in part by modifying mitochondrial respiratory activity. Additionally, mitochondrial efficiency is one plausible mechanism underlying the differing physiological responses to β-AA across livestock species.
Metabolism
Supplementation with artificial sweetener and capsaicin alters metabolic flexibility and performance in heat-stressed and feed-restricted pigs
A combined capsaicin and artificial sweetener supplement provided to growing pigs during heat stress maintained metabolic flexibility, a measure of substrate preference, and metabolic health. Supplementation also improved feed efficiency and weight gain in feed-restricted animals.
Poor maternal diet during gestation alters offspring muscle proteome in sheep
The abundance of proteins related to protein synthesis and degradation, stress responses, metabolism, and oxidative stress are altered in the longissimus from offspring of poorly nourished dams.
Primary myoblasts from intrauterine growth-restricted fetal sheep exhibit intrinsic dysfunction of proliferation and differentiation that coincides with enrichment of inflammatory cytokine signaling pathways
Mechanisms underlying the link between low birth weight and poor muscle growth must be identified in order to improve outcomes and recover value in low birth weight livestock. This study shows that one mechanism is the enhancement of inflammatory regulation of the stem cells responsible for muscle growth.
Elevated dietary selenium rescues mitochondrial capacity impairment induced by decreased vitamin E intake in young exercising horses
Dietary selenium provided at 0.3 mg/kg dry matter offsets mitochondrial deficits induced by reduced vitamin E intake. Optimal dietary vitamin and mineral requirements must be determined to ensure prevention of fatigue in performance horses.
Short Communication: Supplementation with calcium butyrate causes an increase in the percentage of oxidative fibers in equine gluteus medius muscle
Adult Thoroughbred geldings fed a diet supplemented with calcium butyrate contained a greater percentage of type IIA fibers in the gluteus medius.
The impact of Zearalenone on heat-stressed skeletal muscle in pigs
Exposure to heat stress and mycotoxin is known to cause alterations in growth and production efficiency. Given the likelihood of simultaneous exposure, we examined how these pathological agents separately and collectively impact outcomes of muscle health in swine.
Meat Science
Tandem mass tag labeling to assess proteome differences between intermediate and very tender beef steaks
Predicting and managing meat tenderness is a challenging task for the meat industry, as various factors can influence tenderness. Proteomic profiling provides a global understanding and better insight into the protein changes occurring in postmortem muscle that may potentially impact this important eating quality attribute.
Cattle breed type and anabolic implants impact calpastatin expression and abundance of mRNA associated with protein turnover in the longissimus thoracis of feedlot steers
Anabolic implants and cattle breed type interact with each other to cause changes in mRNA abundance in the longissimus thoracis that are related to protein turnover.
Calpastatin expression of the longissimus thoracis is altered by an interaction between cattle breed type and anabolic implants.
Ruminant Nutrition
Meta-analysis of the effects of the dietary application of exogenous alpha-amylase preparations on performance, nutrient digestibility, and rumen fermentation of lactating dairy cows
• Feeding exogenous alpha-amylases to dairy cows is associated with an increase in starch digestibility, milk protein content, and fat-corrected milk.
• Results from this meta-analysis suggest that lactation stage, dietary starch content, and enzyme dose are the main factors associated with the response to dietary supplementation of alpha-amylases.
• Doses of 600 KNU/kg of exogenous alpha-amylase are associated with a greater starch digestibility, milk yield, and protein content in early lactation dairy cows.
Effect of feeding calcium gluconate embedded in a hydrogenated fat matrix on feed intake, gastrointestinal fermentation and morphology, intestinal brush border enzyme activity and blood metabolites in growing lambs
Dietary strategies that enhance gastrointestinal tract function have potential to improve utilization of nutrients and limit negative interactions with the microbial species colonizing the gastrointestinal tract. This study evaluates the use of hydrogenated fat-embedded calcium gluconate to modulate gastrointestinal tract function.
Application of a hand-held laser methane detector for measuring enteric methane emissions from cattle in intensive farming
The hand-held laser methane detector (LMD) technique has been suggested as a potential method for measuring methane (CH4) emissions from enteric fermentation of ruminants in the field. The LMD method can be applied to evaluate differences in CH4 emissions in cattle efficiently using the protocol established in this study.
TECHNICAL NOTE: Analysis of volatile fatty acids in rumen fluid by gas chromatography mass spectrometry using a dimethyl carbonate extraction
Using dimethyl carbonate to extract volatile fatty acids from rumen fluid provides a method that can be used to analyze volatile fatty acids using gas chromatography with a mass spectrometer detector. This method avoids hazardous chemicals commonly used in other analytical procedures.
Non Ruminant Nutrition
Effect of live yeast supplementation in sow diet during gestation and lactation on sow and piglet fecal microbiota, health, and performance
Feeding sows with Saccharomyces cerevisiae var. boulardii during gestation and lactation affected the fecal microbiota of piglets up to weaning but changed neither performance nor health of piglets around weaning.
Postbiotic effects of Lactobacillus fermentate on intestinal health, mucosa-associated microbiota, and growth efficiency of nursery pigs challenged with F18+Escherichia coli
Lactobacillus fermentate effectively enhanced intestinal microbiota diversity and intestinal health of newly weaned pigs challenged with F18+Escherichia coli fed an antibiotic-free diet.
Short Communications
Maternal undernutrition during periconceptional period affects whole-genome ovine muscle methylation in adult offspring
Alteration of the maternal diet of sheep impacts the CpG methylation of genes related to muscular tissue development in muscle tissue of adult offspring.
Animal Genetics and Genomics
Improvement in feed efficiency and reduction in nutrient loading from rainbow trout farms: the role of selective breeding
This study shows that the feed conversion ratio of farmed rainbow trout has been improved by selective breeding, and hence, the same amount of seafood can be produced with significantly reduced amounts of raw materials and reduced environmental impact.
Genetic and phenotypic parameters for feed efficiency and component traits in American mink
This study provides a comprehensive estimation of genetic and phenotypic parameters of feed efficiency traits in American mink, by which mink breeders can develop a selection program to select for feed-efficient mink.
Animal Health and Well Being
Technical Note: A procedure to place urinary catheters in 1- and 6-week-old preweaned Holstein heifer calves for the in vivo evaluation of intestinal permeability
Urethral catheterization can be successfully applied to evaluate gastrointestinal tract permeability in 1- and 6-wk-old Holstein heifer calves.
Companion Animal Nutrition
Sensory attributes, dog preference ranking, and oxidation rate evaluation of sorghum-based baked treats supplemented with soluble animal proteins
This work indicated that the replacement of WWF-GTN by WWS and WRS, along with soluble animal proteins SDP or GL would produce comparable preference by dogs, oxidation rates, product aromatics, flavor, and aftertaste attributes, and, at a lower degree, product texture.
Oral microbiota populations of adult dogs consuming wet or dry foods
Twelve healthy adult dogs were used to determine differences in oral health measures, breath odor, and oral bacteria populations of dogs consuming wet or dry foods. Our results suggest that dogs consuming dry foods have lower breath odor, less tooth plaque buildup, and an oral microbiota population more closely associated with health than dogs consuming wet foods.
Molecular Nutrition
Increased supply of methionine during a heat-stress challenge in lactating holstein cows alters mammary tissue mTOR signaling and its response to lipopolysaccharide
Enhanced post-ruminal supply of methionine can alter mammary metabolism and immune response through transcriptional and post-translational mechanisms.
Immunology
Impact of prepartum administration of a vaccine against infectious calf diarrhea on nonspecific colostral immunoglobulin concentrations of dairy cows
We used novel techniques to show that prepartum vaccination of dairy cows with a calf diarrhea vaccine increased colostral concentrations of not only vaccine-specific immunoglobulins but also non-vaccine-specific immunoglobulin M. More work is required to confirm these preliminary findings, whether the effect may occur in other immunoglobulin classes, and whether this may be a new tool for improving calf health and productivity through enhanced colostrum quality.
Meat Science
Differences in carcass chilling rate underlie differences in sensory traits of pork chops from pigs with heavier carcass weights
Heavier pork carcasses produce more tender pork. This has been demonstrated previously. However, these data suggest that slower chilling rates accompany heavier pork carcasses particularly during the conversion of muscle to meat. Hot carcass weight explained a significant portion of the variation in chilling rate during the first 5 h postmortem, and carcass temperature at 5 h postmortem explained a significant portion of the variation in instrumental tenderness.