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Cynthia Jinno, Kwangwook Kim, Minho Song, Peng Ji, Elizabeth Maga, Yanhong Liu, 141 Supplementation of Bacillus subtilis modified fecal microbiota of weaning pigs experimentally infected with pathogenic E. coli, Journal of Animal Science, Volume 97, Issue Supplement_2, July 2019, Page 81, https://doi.org/10.1093/jas/skz122.147
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Abstract
The objective of this experiment was to investigate the influence of Bacillus subtilis supplements on fecal microbiota of pigs experimentally infected with F-18 E. coli. Forty-eight pigs (6.73 ± 0.77 kg BW) were individually housed in disease containment rooms and randomly allotted to one of four treatments with 12 replicate pigs per treatment. Four treatments included negative control (NC), positive control (PC), single dose probiotics, and double dose probiotics. Pigs in the NC and PC groups were fed with basal diet but without or with E. coli challenge, respectively. Pigs in the probiotics groups were fed the diets either supplemented with 1.28 × 109 CFU or 2.56 × 109 CFU Bacillus subtilis/kg feed and challenged with E. coli. The experiment lasted 18 d with 7 d before and 11 d after the first E. coli inoculation (d 0). Fecal samples were collected on d 5 and d 12 post inoculation (PI) and fecal microbiota was analyzed using 16S rRNA gene sequencing at the V4 hypervariable region and compositional data was analyzed using QIIME2 (2016.6). Pigs in the NC group had less (P < 0.05) Shannon diversity index than pigs in the other groups on d 5 PI, but no difference was observed in Shannon diversity among all treatment groups on d 12 PI. Bray Curtis PCoA analysis displayed that pigs in both probiotics group were separately clustered from the NC and PC groups on d 5 PI. Supplementation of single or double dose probiotics reduced (P < 0.05) the abundance of fecal Firmicutes compared with NC and PC. Within this phylum, the abundance of Lachnospiraceae was increased (P < 0.05), but the abundance of Lactobacillaceae and Streptococcaceae was decreased (P < 0.05). In conclusion, supplementation of Bacillus subtilis could modify gut microbiota of weaned pigs challenged with F18 E. coli.
