Abstract

PROP1 is expressed in the pituitary gland, and plays an important role in the morphogenesis of the pituitary gonadotropes as well as lactotropes, somatotropes and caudomedial thyrotropes. It also controls the expression of growth hormone (GH) prolactin (PRL) and thyroid stimulating hormone (TSH) subunits, through regulatory PIT1 factor. STAT5A was discovered initially as a PRL-induced transcription factor. It is a key intracellular mediator of prolactin signaling and can activate transcription of milk protein genes in response to prolactin. Therefore, PROP1 and STAT5A genes could be considered as a candidate gene for milk production. PCR-RFLP methods were used to identify 2 SNPs: g.6852C > T in STAT5A gene and g.1795C > T in PROP1 gene. For these SNPs a preliminary association analysis with milk production traits was performed by using phenotypic measurements (milk yield, protein and fat content, somatic cell score) obtained from 100 lactating goats. The statistical results showed significant associations for PROP1 and somatic cell score. Further analyses, including larger goat’s populations, are needed to understand the possible relationship between the STAT5A and PROP1genes and milk production traits in Czech national dairy goat (White Shorthaired and Brown Shorthaired goats).

This work was supported by the project no. NAZV QJ1510137 and MZE RO0716.

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