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Andrea Isaák, Péter Gergely, Zsuzsanna Szekeres, József Prechl, Gyula Poór, Anna Erdei, János Gergely, Physiological up-regulation of inhibitory receptors FcγRII and CR1 on memory B cells is lacking in SLE patients, International Immunology, Volume 20, Issue 2, February 2008, Pages 185–192, https://doi.org/10.1093/intimm/dxm132
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Abstract
Under physiological conditions immune complexes (IC) are efficiently cleared from the circulation and meanwhile provide important feedback signals for the immune system via FcγRs and complement receptors. Dysregulation of these mechanisms have been implicated in conditions where IC concentrations reach pathological levels and inflict diseases, like systemic lupus erythematosus (SLE). Our aim was to compare distinct sub-populations of CD19+ B cells of healthy individuals and SLE patients with regard to their expression of FcγR type II (FcγRII, CD32), complement receptor type 1 (CR1, CD35) and complement receptor type 2 (CR2, CD21) and sIgG/IgM. The following four groups of peripheral CD19+ B cells were investigated: IgM+/CD27− naive, IgM+/CD27+ and IgM−/CD27+ memory cells and CD27high plasmablasts. We demonstrate that the expression of the inhibitory receptors FcγRII and CR1 is up-regulated on peripheral memory B cells of healthy controls, whereas this up-regulation is considerably impaired on the memory B cells of SLE patients. This reduction affects both the IgM+ and switched memory B cells. We found a striking difference between the expression of complement receptors CD21 and CD35; namely, no up-regulation of CD21 occurred on the memory B cells of healthy donors, and its decreased expression in SLE patients was characteristic for both the CD27− naive and the CD27+ memory B-cell populations. Our results clearly demonstrate that the previously reported reduced expression of IC-binding receptors is mainly due to the disturbed memory compartment; however, the higher frequency of CD19+/CD27high/sIglow plasmablasts expressing minimal levels of these receptors also contributes to this diminution.
