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G. Grizard, N. Lombard-Vignon, D. Boucher, Changes in carnitine and acetylcarnitine in human semen during cryopreservation, Human Reproduction, Volume 7, Issue 9, 1 October 1992, Pages 1245–1248, https://doi.org/10.1093/oxfordjournals.humrep.a137835
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Abstract
L-Camitine and acetylcamitine concentrations were determined in spermatozoa and seminal plasma from 15 men, in both fresh ejaculate and frozen-thawed semen with cryopro-tective medium. Sperm motility was also evaluated. In fresh samples, the levels of carnhine and acetykarnitine in seminal plasma were comparable whereas in spermatozoa, acetylcamitine predominated. Cryopreservatkm did not change the carnitine and acetylcamitine levels in seminal plasma nor the camitine concentration in spermatozoa; by contrast, the acetylcamitine level in spermatozoa was decreased in 14 cases (110 ± 8 versus 210 ± 20 nmol/108 cells). This decrease in acetylcamitine content was greater during semen dilution in cryoprotectant than after the freezing/thawing process. Motility was also decreased in all cases after the freezing/ thawing process. These results suggest that acetykarnitine recovery in spermatozoa is further evidence of the deleterious effect of the cryoprotective medium in the cryopreservation of semen.