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Li Rong-Hao, Shu-yi Luo, Lin-Zhi Zhuang, Establishment and characterization of a cytotrophoblast cell line from normal placenta of human origin, Human Reproduction, Volume 11, Issue 6, 1 June 1996, Pages 1328–1333, https://doi.org/10.1093/oxfordjournals.humrep.a019381
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Abstract
A cell line has been established from human placentae at the first trimester of normal pregnancy. The cell line was obtained by culture of purified cytotrophoblast cells in serum-free medium supplemented with epidermal growth factor, insulin, dexamethasone and 0.1% bovine serum albumin. The cells can be subcultured for >30 passages in one to three splits. All the cells were mononuclear epithelial-like cells positive to cytokeratin 18, gonadotrophin-releasing . hormone (GnRH), neuropeptide Y, neurotensin, leucine-enkephalin, dopamine and 5-hydroxytryptamine inununo-cytochemical staining. The cells secreted GnRH, progesterone and oestradiol (in the presence of testosterone) but little human chorionic gonadotrophin and no β-endorphin. The cell line showed human karyotypes and had a population doubling time of 48 h in serum-free medium. However, the cells would stop growing in the medium containing fetal bovine serum. A normal cytotrophoblast cell line established in serum-free medium will be particularly useful in the study of cytotrophoblast cell proliferation and differentiation.
- dopamine
- testosterone
- serotonin
- karyotype determination procedure
- cell proliferation
- chorionic gonadotropin
- dexamethasone
- epithelium
- estradiol
- gonadotropins
- gonadotropin-releasing hormone
- hormones
- cattle
- cell lines
- endorphins
- enkephalin, leucine
- epidermal growth factor
- fetus
- keratins
- neuropeptide y
- neurotensin
- pregnancy trimester, first
- serum albumin, bovine
- insulin
- placenta
- progesterone
- normal pregnancy
- cytotrophoblast
- doubling time