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Summary

The genes encoding urease activity of Klebsiella pneumoniae were cloned and expressed in Escherichia coli. Transformants containing recombinant plasmids were selected by the antibiotic resistance phenotype and the production of urease in a Urease-test agar. Deletion derivatives of the parental recombinant plasmid were construced, and the relative position of six genes, necessary for urease production, was determined. Using a colorimetric assay it was demonstrated that some of the transformants exhibited ureolytic activity up to six-times greater than that of the original K pneumoniae isolate. Dot-blot DNA hybridization analysis revealed that the urease gene cluster of K. pneumoniae possesses no significant homology with those of Proteus species and Morganella morganii.

Recombinant DNA, Plasmid, Urease, Gene cluster

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© 1988 Federation of European Microbiological Societies
Issue Section:
Research letter
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