Convenient construction of strains useful for transducing recA mutations with bacteriophage P1 Free

The generalized transducing phage P1 grew well on heterozygous Escherichia coli carrying recA srlC300::Tn10 on the chromosome and recA⁺ on a pBR322-derived plasmid. Because of the close linkage of Tn10 to recA mutations, including recA1, recA13, recA56, recA deletion and recA allele of E. coli BNG30, the latter can be moved to other strains in transductional crosses for selective resistance to tetracycline.