The role of mitochondria in the uptake of methyltriphenylphosphonium ion by Saccharomyces cerevisiae Free

The uptake of a membrane potential (Δψ) probe by strain D27 of Saccharomyces cerevisiae has been shown to have properties consistent with entry occurring by means of a non-mediated process rather than the thiamine transporter. Experiments in which the mitochondrial membrane was selectively depolarised indicated that the probe used partitioned into mitochondria thus limiting its usefulness for quantitative measurements of Δψ across the cytoplasmic membrane.