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Summary

The genes responsible for the formation of F9 fimbriae of the uropathogenic Escherichia coli strain C1018 were cloned by a cosmid cloning procedure. A positive clone was further subcloned by removing two Bam HI fragments and the remaining plasmid pPIL288-10 had a size of 25 kb. This clone still produced fimbriae as judged by electron microscopy and mannose-resistant haemagglutination (MRHA). Antisera were raised against the clone and against fimbriae purified from the clone. The first antiserum was used in a Western blot to prove the purity of the F9 fimbriae. The antiserum raised against purified fimbriae was used in inhibition tests of MRHA and adherence of cloned bacteria to human uroepithelial cells.

Cosmids, Western blotting, adherence, mannose resistant haemagglutination

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© 1985 Federation of European Microbiological Societie
Issue Section:
Research letter
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