-
Views
-
Cite
Cite
Anja Rothkamp, Birgit Strommenger, Gerald-F. Gerlach, Identification of Brachyspira hyodysenteriae-specific DNA fragments using representational difference analysis, FEMS Microbiology Letters, Volume 210, Issue 2, May 2002, Pages 173–179, https://doi.org/10.1111/j.1574-6968.2002.tb11177.x
- Share Icon Share
Abstract
Two novel Brachyspira hyodysenteriae-specific DNA fragments, designated as Bh100 and Bh400, were identified using representational difference analysis. To isolate the fragments the combined DNA of the Brachyspira pilosicoli, Brachyspira intermedia, Brachyspira murdochii and Brachyspira innocens reference strains was subtracted from the genome of B. hyodysenteriae strain B204. Both fragments were present in a single copy and mapped to different positions on the genome of B. hyodysenteriae B78T. Larger fragments encompassing the continuous open reading frames (ORF) of Bh100 and Bh400 were cloned and analysed. Whereas the ORF of 2130 bp encompassing Bh100 did not show homology to any known bacterial protein, Bh400 was part of a putative operon with significant homology to the phosphotransferase system of Bacillus subtilis.