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Sacha A.F.T. van Hijum, Kees Bonting, Marc J.E.C. van der Maarel, Lubbert Dijkhuizen, Purification of a novel fructosyltransferase from Lactobacillus reuteri strain 121 and characterization of the levan produced, FEMS Microbiology Letters, Volume 205, Issue 2, December 2001, Pages 323–328, https://doi.org/10.1111/j.1574-6968.2001.tb10967.x
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Abstract
Fructosyltransferase (FTF) enzymes have been characterized from various Gram-positive bacteria, but not from Lactobacillus sp. In a screening of 182 lactobacilli for polysaccharide production only one strain, Lactobacillus reuteri strain 121, was found to produce a fructan being a levan. Here we report the first-time identification and biochemical characterization of a Lactobacillus FTF enzyme. When incubated with sucrose the enzyme produced a levan that is identical to that produced by Lb. reuteri strain 121 cells.