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Xuanlin Tu, Israel Nisan, Jeff F. Miller, Emanuel Hanski, Ilan Rosenshine, Construction of mini-Tn5cyaA′ and its utilization for the identification of genes encoding surface-exposed and secreted proteins in Bordetella bronchiseptica, FEMS Microbiology Letters, Volume 205, Issue 1, November 2001, Pages 119–123, https://doi.org/10.1111/j.1574-6968.2001.tb10934.x
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Abstract
A mini-Tn5 transposon derivative, mini-Tn5cyaA′, has been constructed. It contains a promoter-less and ribosome binding site-deficient reporter gene, encoding the catalytic domain of Bordetella pertussis adenylate cyclase toxin (CyaA′). We used this system to mutagenize B. bronchiseptica and we developed a screen for identification of mutants containing cyaA′ translational fusions. This system was used to identify B. bronchiseptica genes that encode surface-exposed and secreted proteins.