Abstract

A mini-Tn5 transposon derivative, mini-Tn5cyaA′, has been constructed. It contains a promoter-less and ribosome binding site-deficient reporter gene, encoding the catalytic domain of Bordetella pertussis adenylate cyclase toxin (CyaA′). We used this system to mutagenize B. bronchiseptica and we developed a screen for identification of mutants containing cyaA′ translational fusions. This system was used to identify B. bronchiseptica genes that encode surface-exposed and secreted proteins.

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