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Kelly Edwards, Irina Linetsky, Christopher Hueser, Abraham Eisenstark, Genetic variability among archival cultures of Salmonella typhimurium, FEMS Microbiology Letters, Volume 199, Issue 2, May 2001, Pages 215–219, https://doi.org/10.1111/j.1574-6968.2001.tb10677.x
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Abstract
The existence in our laboratory of over 10 000 Salmonella typhimurium LT2 cultures sealed in agar stab vials for 33–46 years offers an opportunity for evolutionary and mutational studies. In each of 77 vials examined, 103–105 colony forming units per vial were recovered (less than 0.01% of the original population) even after decades of undisturbed storage. Considerable genetic variability was observed in these populations. Three genetic variables, chromosome fragment size as determined by pulsed-field gel electrophoresis, extensive mutational reversions from nutritional auxotrophy to prototrophy, and differences in protein content as assayed by sodium dodecyl sulfate polyacrylamide gel electrophoresis, were measured.