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Hideaki Nanamiya, Nobutake Fugono, Kei Asai, Roy H Doi, Fujio Kawamura, Suppression of temperature-sensitive sporulation mutation in the Bacillus subtilis sigA gene by rpoB mutation, FEMS Microbiology Letters, Volume 192, Issue 2, November 2000, Pages 237–241, https://doi.org/10.1111/j.1574-6968.2000.tb09388.x
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Abstract
We isolated a temperature-sensitive sporulation defective mutant of the sigA gene, encoding a major sigma factor, σA protein, in Bacillus subtilis, and designated it as sigA21. The sigA21 mutation caused a single-amino acid substitution, E314K, in region 4 of the σA protein. In this mutant, expression of the spoIIG gene, whose transcription depends on both σA and the phosphorylated Spo0A protein, Spo0A∼P, a major transcription factor during early stages of sporulation, was greatly reduced at 43°C. To obtain further information on the mechanism of σA function during the early spore development, we isolated a spontaneous sporulation-proficient suppressor mutant at 43°C. This extragenic suppressor mutation was mapped within the rpoB gene, encoding the β subunit of RNA polymerase, and was found to have a single-amino acid substitution, A863G. In this mutant, the expression of the spoIIG is partially restored at 43°C.
