-
Views
-
Cite
Cite
Leo Eberl, Michael Givskov, Lars Kongsbak Poulsen, Søren Molin, Use of bioluminescence for monitoring the viability of individual Pseudomonas putida KT2442 cells, FEMS Microbiology Letters, Volume 149, Issue 1, April 1997, Pages 133–140, https://doi.org/10.1111/j.1574-6968.1997.tb10320.x
- Share Icon Share
Abstract
Exponential phase cells of Pseudomonas putida KT2442 rapidly lost viability when incubated at 0°C without entering a viable but non-culturable state. The majority of dead cells retained their cellular integrity and contained DNA. However, their cellular rRNA content was substantially reduced. By employing a luciferase-marked derivative of P. putida KT2442 in combination with a highly sensitive low-light imaging system, live and dead cells could be distinguished.