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Rustern I. Aminov, Takafumi Nagamine, Koretsugu Ogata, Mutsumi Sugiura, Kiyoshi Tajima, Yoshimi Benno, Cloning, sequencing and complementation analysis of the recA gene from Prevotella ruminicola, FEMS Microbiology Letters, Volume 144, Issue 1, October 1996, Pages 53–59, https://doi.org/10.1111/j.1574-6968.1996.tb08508.x
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Abstract
Degenerate PCR primers based on conserved RecA protein regions were used to amplify a portion of recE from Prevotella ruminicola strain 23, which was used as a probe to isolate the full-length recA gene from the P. ruminicola genomic library. The P. ruminicola recA gene encoded a protein of 340 amino acids with a molecular mass of 36.81 kDa. P. ruminicola RecA was highly similar to other RecA proteins and most closely resembled that of Bacteroides fragilis (75% identity). It alleviated the methyl methanesulfonate and mitomycin C sensitivities of Escherichia coli recA mutants, but did not restore the resistance to UV-light irradiation. Mitomycin C treatment of otherwise isogenic E. coli strains showed a higher level of prophage induction in a recA harboring lysogen.