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Abstract

To study the correlation between emetic toxin and HEp-2 vacuole activity produced by Bacillus cereus isolated from an outbreak of vomiting-type food poisoning, some properties and emetic activities of both purified HEp-2 factor (cereulide) and partially purified factor to rhesus monkeys were determined. The results indicate that both cereulide and partially purified factor were very stable to digestion with proteolytic enzymes, different pH, and heating. Vomiting was induced in the rhesus monkeys orally administered with both substances. From these findings, cereulide (or HEp-2 vacuole factor) is strongly suggested to be an emetic toxin itself.

Bacillus cereus, Emesis, HEp-2 cells, Rhesus monkey, Vacuolation factor

REFERENCES

[1]

Turnbull
P.C.B.
Kramer
J.M.
Jorgensen
K.
Gilbert
R.J.
Melling
J.
Properties and producion characteristics of vomiting, and necrotizing toxins of Bacillus cereus
1979
Am. J. Clin. Nutr.
,
32
,
219
–
228
.

PubMed
 
[2]

Thompson
N.E.
Ketterhagen
M.J.
Bergdoll
M.S.
Schantz
E.J.
Isolation and some properties of an enterotoxin produced by Bacillus cereus
1984
Infect. Immun.
,
43
,
887
–
894
.

PubMed
 
[3]

Gilbert
R.J.
Kramer
J.M.
Bacillus cerus enterotoxin: present status
1984
Biochem. Soc. Trans.
,
12
,
198
–
200
.

Crossref
Search ADS
PubMed
 
[4]

Shinagawa
K.
Ueno
S.
Matsusaka
N.
Sugii
S.
In vitro stability in biological activity and antigenicity of the vascular permeability factor produced by Bacillus cereus
1991
J. Vet. Med. Sci.
,
53
,
317
–
319
.

Crossref
Search ADS
PubMed
 
[5]

Shinagawa
K.
Sugiyama
J.
Terada
T.
Matsusaka
N.
Sugii
S.
Improved methods for purification of an enterotoxin produced by Bacillus cereus
1991
FEMS Microbiol. Lett.
,
80
,
1
–
6
.

Crossref
Search ADS
 
[6]

Granum
P.E.
Brynestad
S.
O'Sullivan
K.
Nissen
H.
Enterotoxin from Bacillus cerus: production and biochemical characterization
1993
Neth. Milk Dairy J.
,
47
,
63
–
70
.

 
[7]

Bennett
R.J.
Murthy
G.
Kaylor
L.
Cox
S.
Harmon
S.M.
Biological characterization and serological identification of Bacillus cereus diarrhoeal factor
1993
Neth. Milk Dairy J.
,
47
,
105
–
120
.

 
[8]

Melling
J.
Capel
B.J.
Characteristics of Bacillus cereus emetic toxin
1978
FEMS Microbiol. Lett.
,
4
,
133
–
135
.

Crossref
Search ADS
 
[9]

Melling
J.
Capel
B.J.
Turnbull
P.C.B.
Gilbert
R.J.
Identification of a novel enterotoxigenic activity associated with Bacillus cereus
1976
J. Clin. Pathol.
,
29
,
938
–
940
.

Crossref
Search ADS
PubMed
 
[10]

Hughes
S.
Bartholomew
B.
Hardy
J.C.
Kramer
J.M.
Potential application of a HEp-2 cell assay in the investigation of Bacillus cereus emetic-syndrome food poisoning
1988
FEMS Microbiol. Lett.
,
52
,
7
–
11
.

Crossref
Search ADS
 
[11]

Shinagawa
K.
Otake
S.
Matsusaka
N.
Sugii
S.
Production of the vacuolation factor of Bacillus cerus isolated from vomiting-type food poisoning
1992
J. Vet. Med. Sci.
,
54
,
443
–
446
.

Crossref
Search ADS
PubMed
 
[12]

Agata
N.
Mori
M.
Ohta
M.
Suwan
S.
Ohtani
I.
Isobe
M.
A novel dodecadepsipeptide, cereulide, isolated from Bacillus cereus causes vacuole formation in Hep-2 cells
1994
FEMS Microbiol. Lett.
,
121
,
31
–
34
.

PubMed
 
[13]

Shinagawa
K.
Kunita
N.
Sasaki
Y.
Okamoto
A.
Biochemical characteristics and heat tolerance of strains of Bacillus cereus isolated from uncooked rice and cooked rice after food poisoning outbreaks (in Japanese with English summary)
1979
J. Food Hyg. Soc. Japan
,
20
,
431
–
436
.

Crossref
Search ADS
 
[14]

Shinagawa
K.
Matsusaka
N.
Konuma
H.
Kurata
H.
The relation between the diarrheal and other biological activities of Bacillus cereus involved in food poisoning putbreaks
1985
Jpn. J. Vet. Sci.
,
47
,
557
–
565
.

Crossref
Search ADS
 
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© 1995 Federation of European Microbiological Societies. All rights reserved
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