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Young Investigators' Award Session, European journal of cardiovascular prevention and rehabilitation, Volume 16, Issue 1_suppl, 1 May 2009, Pages S121–S122, https://doi.org/10.1177/17418267090160s117
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Cardiac microvascular dysfunctionin diabetes and insulin treatment: Role of glucose-induced PKC-BII activity
ZY Yin1, LP Wei1, HC Wang1
1Xijing Hospital, Xi'an, China, People's Republic of
Topic: Heart disease
Purpose: Diabetes mellitus is an independent risk factor for cardiovascular disease and little attention is addressed on PKC-βII in cardiac microvascular dysfunction.
Methods: In animal experiment, normal Sprague-Dawley rat, streptozotocin-induced diabetic rat, insulin-treated and physiological saline-treated diabetic rat were administrated with a serial of evaluations including pressure measurements, angiogenesis and permeability observations under electron microscope, histopathologic analysis for cardiac microvascular endothelium cell (CMECs), TUNEL, and Western blotting for PKC-βII. In cell research part, CMECs in four different mediums (normal medium, high-glucose concentration medium, insulin-stimulated and physiological saline-stimulated high-glucose medium) were investigated with MTT, apoptosis, quantitative permeability assessment and Western blotting.
Results: 1. Accompanied with more active expression of PKC-βII and higher apoptosis rate in diabetic model, either increased microvascular permeability or pathological angiogenesis is observed, and which is attenuated in certain extent while receiving insulin treatment. 2. Accordant results from cell research were obtained. Compared with normal group, CMECs in high-glucose medium are demonstrated with poor proliferation, more notable apoptosis, increased permeability of cell monolayer, and augmented PKC-βII expression. Insulin-stimulated group poses a midst performance between normal and high-glucose group.
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