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M. Kato, S. Yamashina, N. Takeda, S. Mochizuki, T. Morishita, M. Nagano, Molecular biological and quantitative abnormalities of ADP/ATP carrier protein in cardiomyopathic hamsters, European Heart Journal, Volume 16, Issue suppl_O, December 1995, Pages 78–80, https://doi.org/10.1093/eurheartj/16.suppl_O.78
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Abstract
The adenine nucleotide translocator or ADP/ATP carrier protein (AAC) is an integral protein present in the inner mito-chondrial membrane, which performs the exchange of cytoplasmic and intramitochondrial ADP and ATP. The myocardial AAC content was studied in J-2-N cardiomyopathic hamsters. The AAC content was found to be significantly decreased in J-2-N hamsters. For molecular biological analysis, hamster AAC (TI isoenzyme) cDNA was cloned by the plaque hybridization method. This AAC cDNA hybridized specifically with AAC mRNA, so RNA dot-blot hybridization was performed. The highest AAC mRNA level was observed in control hamsters followed by J-2-N hamsters with mild myocardial damage, J-2-N hamsters with severe myocardial damage and Bio 14-6 cardiomyopathic hamsters. These results suggest that a decreased AAC content may contribute to the pathogenesis of cardiomyopathy and that a decrease of AAC mRNA levels may explain the abnormalities of AAC in J-2-N cardiomyopathic hamsters.