Abstract

Antigenic mutants of type 3 poliovirus were obtained by picking plaques formed in the presence of neutralizing monoclonal antibodies. All the mutants were clustered into a single operationally defined antigenic site based on their reactions with a panel of antibodies. Most of the mutations were shown to be located in the virion protein VPl by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and oligonucleotide mappmg, and 15 of 16 mutants. had single-point mutations in a region of VPl encompassing only eight ammo acids, a finding suggesting that this is a major target for the neutralization of the virus. Whereas other areas may also be required, it is likely that this region is a necessary component of a vaccine of any kind.

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