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To the Editor—The emergence and cases of clinical infection of New Delhi metallo-β-lactamase 1 (NDM-1) have been reported in many countries and have caused serious concern worldwide [1–5]. Gut flora is the largest reservoir of resistant genes [6–8], so intestinal carriage is a key factor in NDM-1 infection epidemiology. To investigate the infection status of NDM-1–producing bacteria in humans, from January to October 2011, a total of 10 273 clinical fecal samples were collected from separate individuals in 52 hospitals representing 11 provinces for loop-mediated isothermal amplification (LAMP) and polymerase chain reaction–based detection of blaNDM-1 [9].

Our results showed that the total infection rate of NDM-1–producing intestinal bacteria in clinical patients was 14.8%. For 120 (7.9%) positive samples, a blaNDM-1–possessing strain was isolated and identified. Of the 120 isolates, 52 (43.3%) were stable while the rest were unstable (the blaNDM-1 gene was lost after 2 passages). All of the 120 isolates were resistant to all carbapenems, cephalosporins, and β-lactam inhibitor combinations, and susceptible to colistin. The minimum inhibitory concentration (MIC) for imipenem of most of the 120 isolates was ≥32 μg/mL, but 3 isolates were 8 μg/mL. The stable strains present among 11 species comprising Acinetobacter lwoffii (16 isolates), Kocuria varians (10), Moraxella group (8), Comamonas testosteroni (7), Stenotrophomonas maltophilia (2), Acinetobacter baumannii (2), Morganella morganii (2), Alcaligenes faecalis (2), Staphylococcus capitis (1), Methylobacterium species (1), and Acinetobacter ursingii (1). However no positive isolates were detected among Escherichia coli and Klebsiella pneumoniae, the result was consistent with previous research by Chen et al [10].

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