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Madelon Halula, Francis L. Macrina, Tn5030: A Conjugative Transposon Conferring Clindamycin Resistance in Bacteroides Species, Reviews of Infectious Diseases, Volume 12, Issue Supplement_2, January-February 1990, Pages S235–S242, https://doi.org/10.1093/clinids/12.Supplement_2.S235
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Abstract
Several examples of conjugal transfer of antibiotic resistance determinants in the absence of plasmid DNA have been described in gram-positive species. Previous work had suggested that transfer of clindamycin resistance among the gram-negative anaerobic Bacteroides species might occur by a similar mechanism. Wereport here the characterization of Tn5030, a 45-kb ± 5-kb chromosomal, mobile genetic element devoid of the detectable terminal repeats typically associated with gram-negative transposons. Tn5030 transfers clindamycin resistance (ermFU) and sequences homologous to tetF in the absence of detectable plasmid DNA. This element represents a novel mechanism of genetic exchange among gram-negative organisms.