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N Lahat, H Bitterman, N Yaniv, A Kinarty, N Bitterman, Exposure to hyperbaric oxygen induces tumour necrosis factor-alpha (TNF-α) secretion from rat macrophages, Clinical and Experimental Immunology, Volume 102, Issue 3, December 1995, Pages 655–659, https://doi.org/10.1111/j.1365-2249.1995.tb03867.x
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SUMMARY
We investigated the secretion of TNF-α by monocytes and macrophages derived from the peripheral blood, spleen, and lungs after a single exposure to a therapeutic profile of hyperbaric oxygen (HBO). Rats were exposed for 90 min to either 100% oxygen at 0 28 MPa (2–8 atmospheres absolute) or air. Immediately after exposure, mononuclear cells were isolated from blood, spleen, and lungs and cultured for 18h. The secretion of TNF-α from the cultured monocytes/macrophages was determined with and without stimulation with lipopolysaccharide (LPS). Exposure to hyperbaric oxygen induced a significant increase in the spontaneous ex vivo secretion of TNF-α (without LPS) by mononuclear cells from the blood, spleen, and lung (P < 0 05 from air controls). Stimulation with LPS after exposure to HBO induced a significant increase in TNF-α secretion by lung and spleen macrophages compared with air controls (P<005). However, absolute TNF-α levels were not significantly higher than those achieved ‘spontaneously’ in macrophages exposed to HBO without LPS. Stimulation with LPS induced a marked increase in secretion of TNF-α from blood monocytes after exposure to air, but not after exposure to HBO. These results provide evidence in support of a role played by TNF-α in mediating HBO effects on different tissues and their immune responses.
