The objective of this experiment was to assess the efficacy of conventional and organic insecticides for the control of MBB. Seven synthetic insecticides, four organic insecticides and one untreated check were tested in field plots. The experiment was conducted at Virginia Tech’s Kentland Research Farm in Whitethorne, VA (37.2013° N, −80.5656° W), and consisted of 12 treatments arranged in an RCB design with four replicates. “Caprice” snap beans were planted on 21 May 2015. Plots were 1-row wide and 6.1 m long. Eight rows were planted by hand, at a rate of 40 seeds per meter, with treatments only occurring in the center six rows, creating eight tiers (two tiers per block). Each row was separated by 45 cm of bare soil, and tiers were separated 2 m of bare soil. Foliar treatments were applied on 6, and 14 Jul, with a three-nozzle boom equipped with 8003VS spray tips spaced 20” apart and powered by a CO2 backpack sprayer at 40 psi delivering 30 gpa. All plots were maintained according to standard commercial practices. On 8 (2 DAT1) and 16 (2 DAT2) Jul, five random plants were visually inspected, counting all MBB 3rd and 4th instar larvae. Insect count data did not fit a normal distribution, and therefore, were analyzed using Wilcoxon Test, followed by means separation using Wilcoxon each pair test; significance was accepted at P < 0.05. On 20 Jul (6 DAT2), 50 pods were harvested from each treatment plot and examined for MBB feeding damage. Each pod was rated as either damaged or undamaged, based on the presence of at least 1 cm2 of scared area from feeding. The proportion of damaged pods was calculated per row. Proportion data were normalized using an arcsine square root transformation. Transformed data were analyzed using ANOVA, followed by means separation using Fisher’s LSD; significance was accepted at P < 0.05.

MBB pressure was moderately high in this experiment with the untreated plots averaging 42.5 larvae per 5 plants on the first sample date. At 2 DAT1, Closer SC, Havanta 50SL and Sivanto 200SL treatments had significantly fewer MBB larvae than the untreated check. At 2 DAT2, Radiant SC, Exirel, Havanta 50SL, Sivanto 200SL, Assail 30G and Hero EC treatments had significantly fewer MBB larvae than the untreated check. Exirel, Havanta 50SL, Sivanto 200SL, Assail 30G and Hero EC treatments had significantly fewer MBB larvae than Grandevo and Entrust SC treatments as well. Closer SC, Sivanto 200SL, Assail 30G and Hero EC treatments had significantly fewer MBB damaged pods than the untreated check. Hero EC, Assail 30G, and Sivanto 200SL treatments had fewer MBB damaged pods than Pyganic 1.4EC, Grandevo and Azera treatments as well. Assail 30G, alone, had significantly fewer MBB damaged pods than Exirel, Havanta 50SL and Entrust SC treatments.

Treatment/ formulationRate amt product/acreMean no. MBB larvae/five plants, 2 DAT1Mean no. MBB larvae/five plants, 2 DAT2Mean proportion pods damaged by MBB, 6 DAT2*
Untreated Check42.5±17.7 a20.0±2.1 a0.53±0.03 a
Closer SC2 fl oz3.3±2.0 b12.8±7.4 abc0.38±0.05 bcd
Radiant SC8 fl oz12.0±3.1 ab7.0±3.9 bc0.39±0.02 abcd
Exirel13.5 oz15.0±4.9 a2.8±2.4 c0.44±0.05 abc
Havanta 50SL22 fl oz1.3±0.9 b0.5±0.5 c0.44±0.05 abc
Sivanto 200SL10 fl oz5.5±2.5 b0.3±0.3 c0.34±0.08 cd
Assail 30G5.3 oz7.5 ±3.1 ab0.3±0.3 c0.26±0.02 d
Hero EC10.3 fl oz4.0±4.0 ab0.0±0.0 c0.31±0.04 cd
Grandevo48 oz13.5±2.9 ab20.0±7.8 ab0.49±0.04 ab
Pyganic 1.4 EC17 fl oz9.3±6.0 ab19.3±10.1 abc0.50±0.05 ab
Azera56 fl oz17.5±6.1 ab9.0±5.2 abc0.45±0.06 ab
Entrust SC8 oz13±5.3 ab14.3±2.6 ab0.43±0.07 abc
P value0.0370.00180.016
Treatment/ formulationRate amt product/acreMean no. MBB larvae/five plants, 2 DAT1Mean no. MBB larvae/five plants, 2 DAT2Mean proportion pods damaged by MBB, 6 DAT2*
Untreated Check42.5±17.7 a20.0±2.1 a0.53±0.03 a
Closer SC2 fl oz3.3±2.0 b12.8±7.4 abc0.38±0.05 bcd
Radiant SC8 fl oz12.0±3.1 ab7.0±3.9 bc0.39±0.02 abcd
Exirel13.5 oz15.0±4.9 a2.8±2.4 c0.44±0.05 abc
Havanta 50SL22 fl oz1.3±0.9 b0.5±0.5 c0.44±0.05 abc
Sivanto 200SL10 fl oz5.5±2.5 b0.3±0.3 c0.34±0.08 cd
Assail 30G5.3 oz7.5 ±3.1 ab0.3±0.3 c0.26±0.02 d
Hero EC10.3 fl oz4.0±4.0 ab0.0±0.0 c0.31±0.04 cd
Grandevo48 oz13.5±2.9 ab20.0±7.8 ab0.49±0.04 ab
Pyganic 1.4 EC17 fl oz9.3±6.0 ab19.3±10.1 abc0.50±0.05 ab
Azera56 fl oz17.5±6.1 ab9.0±5.2 abc0.45±0.06 ab
Entrust SC8 oz13±5.3 ab14.3±2.6 ab0.43±0.07 abc
P value0.0370.00180.016

Insect count data were analyzed using a Wilcoxon test. Means were separated using Wilcoxon each pair test at the 0.05 level of significance. Means followed by the same letter within a column are not significantly different.

*

Damaged pod data were analyzed using ANOVA. Means were separated using Fisher’s LSD at the 0.05 level of significance. Means followed by the same letter within a column are not significantly different (P > 0.05).

Treatment/ formulationRate amt product/acreMean no. MBB larvae/five plants, 2 DAT1Mean no. MBB larvae/five plants, 2 DAT2Mean proportion pods damaged by MBB, 6 DAT2*
Untreated Check42.5±17.7 a20.0±2.1 a0.53±0.03 a
Closer SC2 fl oz3.3±2.0 b12.8±7.4 abc0.38±0.05 bcd
Radiant SC8 fl oz12.0±3.1 ab7.0±3.9 bc0.39±0.02 abcd
Exirel13.5 oz15.0±4.9 a2.8±2.4 c0.44±0.05 abc
Havanta 50SL22 fl oz1.3±0.9 b0.5±0.5 c0.44±0.05 abc
Sivanto 200SL10 fl oz5.5±2.5 b0.3±0.3 c0.34±0.08 cd
Assail 30G5.3 oz7.5 ±3.1 ab0.3±0.3 c0.26±0.02 d
Hero EC10.3 fl oz4.0±4.0 ab0.0±0.0 c0.31±0.04 cd
Grandevo48 oz13.5±2.9 ab20.0±7.8 ab0.49±0.04 ab
Pyganic 1.4 EC17 fl oz9.3±6.0 ab19.3±10.1 abc0.50±0.05 ab
Azera56 fl oz17.5±6.1 ab9.0±5.2 abc0.45±0.06 ab
Entrust SC8 oz13±5.3 ab14.3±2.6 ab0.43±0.07 abc
P value0.0370.00180.016
Treatment/ formulationRate amt product/acreMean no. MBB larvae/five plants, 2 DAT1Mean no. MBB larvae/five plants, 2 DAT2Mean proportion pods damaged by MBB, 6 DAT2*
Untreated Check42.5±17.7 a20.0±2.1 a0.53±0.03 a
Closer SC2 fl oz3.3±2.0 b12.8±7.4 abc0.38±0.05 bcd
Radiant SC8 fl oz12.0±3.1 ab7.0±3.9 bc0.39±0.02 abcd
Exirel13.5 oz15.0±4.9 a2.8±2.4 c0.44±0.05 abc
Havanta 50SL22 fl oz1.3±0.9 b0.5±0.5 c0.44±0.05 abc
Sivanto 200SL10 fl oz5.5±2.5 b0.3±0.3 c0.34±0.08 cd
Assail 30G5.3 oz7.5 ±3.1 ab0.3±0.3 c0.26±0.02 d
Hero EC10.3 fl oz4.0±4.0 ab0.0±0.0 c0.31±0.04 cd
Grandevo48 oz13.5±2.9 ab20.0±7.8 ab0.49±0.04 ab
Pyganic 1.4 EC17 fl oz9.3±6.0 ab19.3±10.1 abc0.50±0.05 ab
Azera56 fl oz17.5±6.1 ab9.0±5.2 abc0.45±0.06 ab
Entrust SC8 oz13±5.3 ab14.3±2.6 ab0.43±0.07 abc
P value0.0370.00180.016

Insect count data were analyzed using a Wilcoxon test. Means were separated using Wilcoxon each pair test at the 0.05 level of significance. Means followed by the same letter within a column are not significantly different.

*

Damaged pod data were analyzed using ANOVA. Means were separated using Fisher’s LSD at the 0.05 level of significance. Means followed by the same letter within a column are not significantly different (P > 0.05).

* This research was supported by The Delmarva Land-Grant Institution Seed Grant and industry gifts of pesticides and research funding from Dow Agrosciences, Dupont, MGK, ISK Biosciences, Marrone BioInnovations, FMC, UPI, and Bayer CropScience.

Author notes

Subject Editor: John Palumbo

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