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Abstract

We have developed an automated chemiluminescent immunoassay for quantitating renin in plasma specimens, to be performed on the Nichols Advantage® Specialty System. This assay measures renin directly, is easier to standardize against the WHO reference material (from kidney), and is less labor-intensive than the classical enzyme kinetic PRA and PRC assays. Since the non-proteolytic activation of prorenin is time and temperature dependent, we attempted to minimize this activation by shortening the incubation time (30 min) and raising the incubation temperature to 37°C. Blood samples from normal subjects were obtained during standardized upright and supine positions, and the samples were processed at room temperature to avoid cryoactivation. Results indicate that the renin levels were significantly lower in supine than upright condition. This assay measures renin from 2 to 500 μU/mL with an analytical sensitivity of 1 μU/mL. The assay has no high dose hook for specimens with renin concentrations up to 100,000 μU/mL. Recovery values obtained by serial dilution of EDTA plasma samples were between 87 - 100% across PRA concentrations of 5 - 57 ng/mL/hr. Intra-assay variation of >3 μU/mL is 5%, and inter-assay variation at >3 μU/mL is <10%. Direct comparison of results was made with a validated plasma renin activity (PRA) assay on n=68 patient samples. Range of results was 0.2 - 327 μU/mL for the Nichols Advantage Active Renin assay and 0.3 - 57.7 ng/mL/hr for the PRA assay. Results of linear regression analysis show a correlation coefficient 'r' = 0.97. The Nichols Advantage Active Renin assay is simple and rapid demonstrating good performance over a wide dynamic range.

Active Renin, Chemiluminescence, Automated
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© American Journal of Hypertension, Ltd. 2001
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