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Luis Morcillo, Pedro Aranda, Carlos Maria Ferrario, Javier Aranda, Armando Reyes-Engel, P-356: RAS polymorphisms and angiotensin peptides plasma levels, American Journal of Hypertension, Volume 14, Issue S1, April 2001, Pages 149A–150A, https://doi.org/10.1016/S0895-7061(01)01952-5
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Abstract
The aim of this study has been to analyse the influence of three polymorphisms of the Renin Angiotensin System (RAS), I/D from ACE, M235T from angiotensinogen and a1166c from AT1 receptor, on peptides plasma levels of Angiotensin I, II and 1-7 (AI, AII, A1-7).
It was selected a homogeneous age population (m=20,67, SD=2,75) of 93 healthy subjects (43 men and 50 women). Peptides were separated by HPLC and quantified by RIA.Genotypes were determined by PCR and restriction enzyme analysis.
No differences on peptides plasma levels were found between the nine genotypes in the whole population. However when the subjects were distributed by sex it was observed a significant (p<0.01) higher level of A1-7 in men than women (37.76 vs 26.04 pgr/mL) these differences were maintained in the ratios A1-7/AI and A1-7/AII . Between genotypes those men with T allele had significantly higher A1-7 levels than those with M allele of the angiotensinogen polymorphism. Women with DD genotype showed a non significant increase of AI and AII. Linear correlation between peptides shows significant values between AI/AII and AII/A1-7, but no correlation between AI/A1-7.
There is a sexual dimorphism of A1-7 plasma levels. When the population is distributed by gender it can be noted that genotypes exert influences on angiotensin peptides plasma levels The AII it is showed as a more preferential substrate than AI for A1-7 synthesis.
These differences suggest that sex hormones affect the regulation of the RAS, perhaps renin via, being more clearly noted on M235T polymorphism in men and I/D polymorphism in women.
