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Katsutoshi Takahashi, Katsuyuki Ando, Tamao Otsu, Miki Nagase, Toshiro Fujita, P-341: Dietary potassium prevents renal dysfunction and upregulation of LOX-1 gene in DOCA-salt rats, American Journal of Hypertension, Volume 14, Issue S1, April 2001, Page 143A, https://doi.org/10.1016/S0895-7061(01)01862-3
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Abstract
Objective: To determine the effects of dietary potassium loading on renal function, blood pressure (BP) and oxidative stress in DOCA-salt rats as a model of mineralocorticoid hypertension.
Design and Methods: After unilateral nephrectomy, male 8-week-old Sprague Dawley rats were divided into 3 groups: a) standard chow (0.66% NaCl, 1.34% KCl), b) NaCl-loaded chow (8% NaCl, 1.34% KCl), or c) NaCl and KCl-loaded chow (8% NaCl, 8% KCl). They were weekly subjected to DOCA treatment (100 mg/kg BW). After 2 weeks, BP, plasma and urinary parameters including biomarkers of oxidative stress (plasma 8-epi-PGF2-alfa and 24-hr urinary 8-OH-dG) were determined. Kidneys were subjected to northern blotting to analyze the expression of LOX-1 (lectin-like oxidized LDL receptor-1) gene.
Results: Treatment with DOCA-salt significantly increased systolic BP, associated with renal hypertrophy, renal dysfunction (the increase of s-Cr, proteinuria), and upregulation of LOX-1 gene. Although potassium overload did not have antihypertensive effect, it ameliorated renal dysfunction, renal LOX-1 gene expression, and the increase of markers of oxidative stress in DOCA-salt rats.
Conclusion: These data support the notion that, in mineralocorticoid hypertension, dietary potassium has a protective effect on the renal function independent of BP.
- antihypertensive agents
- northern blotting
- oxidative stress
- proteinuria
- hyperkalemia
- gene expression
- renal function
- systolic blood pressure
- blood pressure
- kidney failure
- biological markers
- dinoprost
- genes
- lectin
- nephrectomy
- plasma
- potassium chloride
- potassium, dietary
- rats, sprague-dawley
- sodium chloride
- up-regulation (physiology)
- urinary tract
- hypertrophy
- kidney
- rats
- mineralocorticoid hypertension
- desoxycorticosterone acetate