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Juan A. Rodriguez-Feo, Juan Gomez, Luis Rico, Santos Casado, Antonio Lopez-Farre, P-315: Cyclic GMP relaxing system in the vascular wall of stroke-prone spontaneously hypertensive rats. Effects of the alpha1-receptor blocker, doxazosin, American Journal of Hypertension, Volume 14, Issue S1, April 2001, Pages 133A–134A, https://doi.org/10.1016/S0895-7061(01)01729-0
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Abstract
The aim of the present study was to analyze in spontaneously hypertensive rats of the stroke-prone substrain (SHRSP) the cyclic GMP (cGMP) relaxing system. SHRSP rats (n=15) demonstrated an impaired relaxing response to the NO-donor sodium nitroprusside (SNP) with respect to normotensive Wistar rats (n=15), that was accompanied by a reduction in the level of the main second messenger of NO, cGMP and a reduced expression of the soluble guanylate cyclase (sGC) beta1-subunit determined by Western blot. Doxazosin (10 mg/Kg bw/day for 15 days, n=15) reduced MAP in SHRSP rats. Doxazosin preserved the endothelium-independent relaxation response to SNP in aortic segments from SHRSP rats and increased the expression of the sGC beta1-subunit. In conclusion, independently of the endothelial NO-generating system, endothelial dysfunction also could result from an impaired signalling functionality in vascular smooth muscle cells. Doxazosin-treatment improved the endothelial-independent relaxation and preserved the cGMP generating system in the smooth muscle cell layer.
- aorta
- doxazosin
- signal transduction
- western blotting
- endothelial dysfunction
- cerebrovascular accident
- ischemic stroke
- cyclic gmp
- muscle, smooth, vascular
- cd29 antigen
- endothelium
- single nucleotide polymorphism
- rats, inbred shr
- rats, wistar
- second messenger systems
- rats
- nitroprusside sodium
- soluble guanylyl cyclase
- myocytes, smooth muscle
- donors